1. Description/Name: Sample preparation for Bio/Geo that will use the low background counting facility

(to be filled out by Onstott/Kieft)

• QUESTION: Do you want separate rooms for bio vs geo or for storage vs prep?

If so, you can make 2 of these lists….

1. Planned use (be specific):
   1. Primary function: Preparation of microbial, solid (like Al foil), gas and liquid samples which have low levels of radioisotope concentrations (equivalent to <~ 1 cpm) for measurement.
   2. Temporary and permanent storage: We will require two -20oC freezers, a refrigerator, and one -80oc freezer.
   3. Sample and detector handling: One wet chemistry bench top for cold Cr extraction - with drawers for phosphor imaging screen exposure if the facility plans to have a phosphor imager. One HEPA filtered hood for RNA extraction.
   4. Jigs, fixtures, scaffolds, etc. necessary - just belts for securing high pressure gas cylinders
   5. E & O activities - none planned at this location - E&O will be associated with biofilm experiments outside FAARM facility
2. Occupancy:
   1. Steady: Anticipate having 2 technicians from different sites around DUSEL processing samples.
   2. Peak: perhaps 5, including post-docs or graduate students.
3. Adjacencies (list/distance): not sure what is meant by this.
4. Space size (gsf and/or WxLxH): 6m x 10m x 3m (I presume any office space is top side)
5. Experiment Systems: GC with gas proportional counter, phosphor imager, multiphoton decay coincidence counter, isotope microarray analyzer (epifluorescent microscope in a dark closet) and beta cage - if this equipment is to be located on the ground floor in the water shield then we can reduce the size of our room.
6. Shielding: what ever is required to achieve 10^-5 counts/KeV-minute - in other words we could operate the equipment within the Bio bay at ambient conditions or make use of one of the counters within the water shield for high sensitivity work.
7. Floor load (PSF): again, nothing special here - ~10 PSF?
8. Material handling (cranes, etc.) - not necessary - fork lift to elevator to get equipment into Bio Bay.
9. Finishes (walls/floor/ceiling): nothing special
10. Casework:
11. Storage: we would probably only keep low-level samples that are actively being counted in the lab along with counting equipment. radiolabeled compounds will not be stored here. measured samples will leave lab for long term storage at surface facility.
12. Furniture/Fixtures/Equipment (non-experiment):
13. Mechanical:
    1. Environmental conditions:
    2. Clean class: 2,000
    3. Ventilation:
    4. Exhaust:
    5. Plumbing (domestic, waste, systems): acid sink
    6. Fire Suppression: acceptable to us
    7. Building Management System:
    8. Fume hoods: one fume hood for acid digestion
    9. Chilled water: no, but D.I. water would be good
    10. Other:
14. Electrical:
    1. Power (clean and dirty): all clean and ~20 amps at 220V.
    2. Lighting: yes - whatever the facility decides
    3. Low Voltage/Data/Communication: yes, but no special requirements
    4. Fire Detection/Alarm: yes
    5. Other:
15. Acoustical: none
16. Vibration Isolation: just a vibration isolation bench for the microscope
17. EMI/RFI Isolation: I don't know what this means.
18. Hazardous materials: Need to be able to dispose of biological samples with low activity for transport to the surface.
19. Special Consideration (expansion, etc.):